Ordering information
Name	Cat. No.	Vol.	Scheme
G-DBCO	PMG035-2	2 ml

1. Overview

Click Chemistry describes rapid, selective "click" reactions between paired functional groups in mild aqueous solutions. This concept has evolved into a convenient, versatile, and reliable two-step coupling procedure for Molecules A and B, widely applied in biosciences, drug discovery, and materials science.

PuriMag™ G-DBCO, Magnetic Beads feature DBCO (Dibenzocyclooctyne) covalently coupled to superparamagnetic nanoparticles. These beads enable rapid, efficient, and spontaneous catalyst-free CLICK chemistry under physiological conditions to capture azide-tagged biomolecules for:

    Labeling & Tracking

    Purification & Isolation

    Protein-Protein/Biomolecule/DNA-Protein interaction studies

Synonyms: DBCO = ADIBO (Azadibenzocyclooctyne) = DIBAC (Dibenzoazacyclooctyne)

Key Mechanism: Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)

    Bioorthogonal reaction between DBCO and azides without copper catalysts

    Minimal off-target labeling in cells/tissue lysates

    Preserves biological integrity during conjugation

Advantages of Click Chemistry:

    High specificity & low background: Inert to native functional groups (e.g., amines)
    Rapid quantitative labeling: Enables non-radioactive analysis of enzymatic activity in vitro/vivo
    Small molecular footprint: Excellent substrate properties

Features of DBCO Beads:

    Covalent capture without cytotoxic catalysts

    Ultra-low non-specific binding after stringent washing

    Compatibility with complex biological matrices

Schematic Diagram of PuriMag™ Bead Coupling Mechanism:

2. product description

Product Specifications
Description	Polymer coated Fe3O4 nanoparticles
Particle Size	200 nm
Number of Beads	~1.7×1010 beads/mg
Matrix	Proprietary polymer
Functional group	DBCO group
Group density	~100 µmole / g of Beads
Magnetization	60~70 EMU/g
Formulation	10mg/mL in DI water
Storage	1 year at 2~8 ℃. Do not freeze.

3. Instructions for Use

    1.Mix ~1 mg DBCO Magnetic Nanoparticles with 60 μM azide-tagged ligand in 100 μL PBS buffer (pH 7.0).

    2.React at 30°C for 2 h with gentle agitation.

    3.Recover the product via magnetic separation.

    4.Wash 3× with PBS (pH 7.0).

        (For research use only!)