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客户采用我司硅羟基磁珠提取COVID-19病毒RNA进行扩增检测在《Biosensors and Bioelectronics》发表论文

2021-6-2 21:48:25点击:

客户采用我公司硅羟基磁珠提取COVID-19新冠病毒RNA,然后不洗脱直接带磁珠进行等温扩增RT-LAMP,用于新冠病毒的检测。

Yugan He, Tie Xie, Yigang Tong,
Rapid and highly sensitive one-tube colorimetric RT-LAMP assay for visual detection of SARS-CoV-2 RNA,
Biosensors and Bioelectronics,
Volume 187,
2021,
113330,
ISSN 0956-5663,
https://doi.org/10.1016/j.bios.2021.113330.
(http://www.sciencedirect.com.group21-s.aronip.com/science/article/pii/S0956566321003675)


Abstract: Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a highly contagious disease. To tame the continuously raging outbreak of COVID-19, developing a cheap, rapid and sensitive testing assay is absolutely imperative. Herein, we developed a one-tube colorimetric RT-LAMP assay for the visual detection of SARS-CoV-2 RNA. The assay integrates Si–OH magnetic beads (MBs)-based fast RNA extraction and rapid isothermal amplification in a single tube, thus bypassing the RNA elution step and directly amplifying on-beads RNA molecules with the visualized results. This one-tube assay has a limit of detection (LOD) as low as 200 copies/mL for sample input volumes of up to 600 μL, and can be performed in less than 1 h from sample collection to result readout. This assay demonstrated a 100% concordance with the gold standard test RT-qPCR test by using 29 clinical specimens and showed high specificity. This one-tube colorimetric RT-LAMP assay can serve as an alternative platform for a rapid and sensitive diagnostic test for COVID-19 and is particularly suitable for use at community clinics or township hospitals.
Keywords: One-tube; SARS-CoV-2; LAMP; Point-of-care test; Visual readout


摘要:冠状病毒病(COVID-19)是由SARS-CoV-2(严重急性呼吸综合征冠状病毒2型)引起的一种高度传染性疾病。为了遏制持续肆虐的COVID-19病毒爆发,开发一种廉价、快速、灵敏的检测方法势在必行。在此,我们开发了一种单管比色RT-LAMP检测SARS-CoV-2rna的方法。该方法将基于硅-羟基磁珠(MBs)的快速RNA提取和快速等温扩增集成在一个试管中,从而绕过RNA洗脱步骤,直接在珠状RNA分子上扩增,结果可视化。这种单管分析的检测限(LOD)低至200拷贝/mL,样品输入量高达600μ五十、 从样品采集到结果读出不到1小时。用29例临床标本进行RT-qPCR检测,结果与金标准试验符合率为100%,具有较高的特异性。这种单管比色RT-LAMP检测方法可以作为COVID-19快速敏感诊断试验的替代平台,特别适合在社区诊所或乡镇医院使用。

关键词:单管;SARS-CoV-2;灯;护理点测试;视觉读数


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