Gefei Li, Wenxiao Ma, Juan Mo, Boyang Cheng, Shin-ichiro Shoda, Demin Zhou*, and Xin-Shan Ye*，Influenza Virus Precision Diagnosis and Continuous Purification Enabled by Neuraminidase-Resistant Glycopolymer-Coated Microbeads，ACS Appl. Mater. Interfaces 2021, 13, 39, 46260–46269，Publication Date:September 22, 2021
https://doi.org/10.1021/acsami.1c11561 （链霉亲和素磁珠偶联寡糖）

Abstract
Rapid diagnosis and vaccine development are critical to prevent the threat posed by viruses. However, rapid tests, such as colloidal gold assays, yield false-negative results due to the low quantities of viruses; moreover, conventional virus purification, including ultracentrifugation and nanofiltration, is multistep and time-consuming, which limits laboratory research and commercial development of viral vaccines. A rapid virus enrichment and purification technique will improve clinical diagnosis sensitivity and simplify vaccine production. Hence, we developed the surface-glycosylated microbeads (glycobeads) featuring chemically synthetic glycoclusters and reversible linkers to selectively capture the influenza virus. The surface plasmon resonance (SPR) evaluation indicated broad spectrum affinity of S-linked glycosides to various influenza viruses. The magnetic glycobeads were integrated into clinical rapid diagnosis, leading to a 30-fold lower limit of detection. Additionally, the captured viruses can be released under physiological conditions, delivering purified viruses with >50% recovery and without decreasing their native infectivity. Notably, this glycobead platform will facilitate the sensitive detection and continuous one-step purification of the target virus that contributes to future vaccine production.

摘要

快速诊断和疫苗开发对于预防病毒构成的威胁至关重要。然而，快速检测，如胶体金检测，由于病毒数量少，会产生假阴性结果。此外，常规病毒纯化，包括超速离心和纳滤，是多步骤且耗时的，这限制了病毒疫苗的实验室研究和商业开发。快速病毒富集和纯化技术将提高临床诊断灵敏度并简化疫苗生产。因此，我们开发了具有化学合成糖簇和可逆接头的表面糖基化微珠（糖磁珠），以选择性捕获流感病毒。表面等离子共振 (SPR) 评估表明 S-连接糖苷对各种流感病毒具有广谱亲和力。磁性糖珠被整合到临床快速诊断中，导致检测下限降低 30 倍。此外，捕获的病毒可以在生理条件下释放，以 > 50% 的回收率提供纯化的病毒，并且不会降低其天然感染性。值得注意的是，该糖珠平台将有助于对有助于未来疫苗生产的目标病毒进行灵敏检测和连续一步纯化。