客户采用我司4um羧基磁珠偶联anti-CD63抗体，然后用于捕获外泌体，裂解后反转率等温扩增，用于检测。


Qiuyuan Lin, Zhipeng Huang, Xin Ye, Bin Yang, Xueen Fang, Baohong Liu, Hui Chen, Jilie Kong,
Lab in a tube: Isolation, extraction, and isothermal amplification detection of exosomal long noncoding RNA of gastric cancer,
Talanta,
Volume 225,
2021,
122090,
ISSN 0039-9140,
https://doi.org/10.1016/j.talanta.2021.122090.
(http://www.sciencedirect.com.group21-s.aronip.com/science/article/pii/S0039914021000114)

Abstract: Tumor-derived exosomes that inherit molecular information on parental cells hold great promise for cancer diagnostics. Currently, two main technical challenges, time-consuming and labor-intensive isolation of exosome and nucleic acid extraction with limited recovery that have restricted the detection of ultralow abundance exosomal nucleic acids. Here, we proposed a simple, efficient and “lab in a tube” system for the detection of exosomal nucleic acids, which fully integrated exosomes enrichment using immunomagnetic beads (IMB) (10 min), fast exosomes lysis based on NP-40 lysate (5 min) and sensitive loop-mediated isothermal amplification (LAMP) in a tube. This method was demonstrated by detecting two exosomal long noncoding RNA biomarkers of gastric cancer (HOTTIP and lncRNA-GC1) with a dynamic detection ranging from 300 ng/μL to 10 ng/μL, and the detection limit of LAMP was 10 ng/μL. Additionally, this platform exhibited good performance in the analysis of exosomal HOTTIP RNA directly in human serum samples, which has the potential for detection of low-abundance exosomal nucleic acid biomarkers from cancers.
Keywords: Exosomal long noncoding RNA; Immunomagnetic beads isolation; Nucleic acid extraction; Loop-mediated isothermal amplification (LAMP)

摘要：肿瘤源性外显子是遗传亲本细胞分子信息的重要来源，对肿瘤诊断具有重要的应用前景。目前，外显子的耗时和劳动密集分离和回收率有限的核酸提取两大技术难题限制了超低丰度外显子核酸的检测。本文提出了一种简便、高效、“试管内实验室”的体外核酸检测系统，该系统利用免疫磁珠（IMB）（10min）、基于NP-40溶解酶（5min）和敏感环介导的等温扩增（LAMP）技术，实现了完整的胞外体富集。本方法通过检测胃癌（HOTTIP和lncRNA-GC1）两个外显体长非编码RNA生物标记物，动态检测范围为300ng，证明了该方法的有效性/μL至10 ng/μ五十、 灯的检测限为10ng/μL。该平台在直接检测人血清外周血中的外周热尖RNA方面表现出良好的性能，有可能检测肿瘤低丰度的外显子核酸生物标志物。

关键词：外显子长非编码RNA；免疫磁珠分离；核酸提取；环介导等温扩增（LAMP）

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