东南大学采用我司蛋白G磁珠偶联抗体捕获细菌进行检测发表论文

Temporal sensing platform based on anodic dissolution of Ag and cathodic biocatalysis of oxygen reduction for Staphylococcus aureus detection

基于Ag阳极溶解和阴极氧还原生物催化金黄色葡萄球菌检测的时间传感平台 

Abstract

An Ag@C hybrid bipolar electrode (BPE) sensing platform has been established for the temporal detection of Staphylococcus aureus (S. aureus) in food. Combining the advantages of anodic dissolution of Ag and cathodic biocatalysis of oxygen (O2) reduction, this strategy showed an ultralow detection limit down to 10 CFU mL−1. As the formation of Ag@C completely quenched the electrochemiluminescence (ECL) emission of luminol, the ECL emission recovery reflected the extent of anodic dissolution. Meanwhile, S. aureus catalyzed the electrochemical reduction of O2 at the cathode, reducing the overpotential for cathodic O2 reduction and thus increasing the rate of anodic electron loss, facilitating Ag dissolution and restoring the ECL emission of luminol. When a constant potential was applied, through monitoring the ECL recovery time before and after the incubation of S. aureus on the cathode, S. aureus could be quantified due to the slight difference of the conductivity.

摘要 

建立了Ag@C混合双极电极（BPE）传感平台，用于食品中金黄色葡萄球菌（S. aureus）的时间检测。结合 Ag 的阳极溶解和氧 (O2) 还原的阴极生物催化的优势，该策略显示出低至 10 CFU mL-1 的检测限。由于 Ag@C 的形成完全淬灭了鲁米诺的电化学发光 (ECL) 发射，因此 ECL 发射恢复反映了阳极溶解的程度。同时，金黄色葡萄球菌在阴极催化了O2的电化学还原，降低了阴极O2还原的过电位，从而增加了阳极电子损失率，促进了Ag的溶解，恢复了鲁米诺的ECL发射。当施加恒定电位时，通过监测金黄色葡萄球菌在阴极上孵育前后的ECL恢复时间，由于电导率的微小差异，可以对金黄色葡萄球菌进行量化。

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