SP3（single-pot, solid-phase-enhanced sample-preparation）是一种基于顺磁颗粒的蛋白质组预处理工作流程，通过有机溶剂乙醇或乙腈驱动驱动蛋白在羧基或其它亲水性磁珠表面被捕获（如图所示）。标准 SP3流程中，蛋白质通过亲水相互作用机制与磁珠结合，结合了蛋白的磁珠被束缚在溶剂化层，可冲洗去除不需要的污染物，然后在一定条件下洗脱纯化的蛋白质。这种亲水捕获机制类似于亲水相互作用色谱（HILIC）中所涉及的机制，已被广泛证明是分离多种生物分子的有效工具。厦门普睿迈格生物科技有限公司开发的SP3-Carboxyl磁珠http://www.purimagbead.com/Product/1736805216.html可以完美替代SP3方案中最常使用的是Cytiva的Sera Mag SpeedBeads（GE Healthcare），提供了测定性能与成本之间最佳平衡。

        以下是Sp3磁珠在蛋白组学样本前处理方面的经典文献，供参考：

1. Hughes C S, Foehr S, Garfield D A, et al. Ultrasensitive proteome analysis using paramagnetic bead technology[J]. Molecular systems biology, 2014, 10(10): 757.
2. Sielaff M, Kuharev J, Bohn T, et al. Evaluation of FASP, SP3, and iST protocols for proteomic sample preparation in the low microgram range[J]. Journal of proteome research, 2017, 16(11): 4060-4072.
   
3. Moggridge S, Sorensen P H, Morin G B, et al. Extending the compatibility of the SP3 paramagnetic bead processing approach for proteomics[J]. Journal of proteome research, 2018, 17(4): 1730-1740.
4. Hughes C S, Moggridge S, Müller T, et al. Single-pot, solid-phase-enhanced sample preparation for proteomics experiments[J]. Nature protocols, 2019, 14(1): 68-85.
5. Hughes C S, Sorensen P H, Morin G B. A standardized and reproducible proteomics protocol for bottom-up quantitative analysis of protein samples using SP3 and mass spectrometry[J]. Proteomics for Biomarker Discovery: Methods and Protocols, 2019: 65-87.
6. Müller T, Kalxdorf M, Longuespée R, et al. Automated sample preparation with SP 3 for low‐input clinical proteomics[J]. Molecular systems biology, 2020, 16(1): e9111.
7. Mikulášek K, Konečná H, Potěšil D, et al. SP3 protocol for proteomic plant sample preparation prior LC-MS/MS[J]. Frontiers in plant science, 2021, 12: 635550.
   
8. Duong V A, Lee H. Bottom-up proteomics: advancements in sample preparation[J]. International Journal of Molecular Sciences, 2023, 24(6): 5350.