湖南大学谭蔚泓院士课题组在Nano Letters 发文采用我司Si-IDA-Ni磁珠结合His标签蛋白

DNA Nanostructure-Programmed Cell Entry via Corner Angle-Mediated Molecular Interaction with Membrane Receptors

Xueyu Peng, Senbiao Fang, Bin Ji, Min Li, Jie Song, Liping Qiu*, and Weihong Tan*
Nano Letters  2021, 21, 16, 6946-6951 (Letter)Subscribed Access
Publication Date (Web):August 16, 2021

DOI: 10.1021/acs.nanolett.1c02191

Despite its polyanionic nature, DNA can cross the negatively charged membrane to enter living cells by assembling into specific nanostructures, establishing various opportunities for biomedical applications. Mechanistic studies to explain how the geometrical parameters of DNA nanostructures impact the cell entry are critical but elusive. Here, we use experimentation and simulation to study the interaction between cells and three typical framework nucleic acids (FNAs), including tetrahedron, triangular prism, and cube. Different cellular uptake efficiency was observed among these FNAs, and similar distinction consistently existed in multiple cell lines. Scavenger receptors (SRs) were demonstrated to be essential in mediating the uptake process. Molecular docking simulations revealed that the SR binding predominantly depended on the corner angle of FNAs, determining cellular internalization frequency. This study clearly explains how FNAs interact with the membrane to initiate cell entry, offering new clues for the design of theranostic nanocarriers and the study of virus invasion.

尽管具有多阴离子性质，但 DNA 可以通过组装成特定的纳米结构穿过带负电荷的膜进入活细胞，从而为生物医学应用创造各种机会。解释 DNA 纳米结构的几何参数如何影响细胞进入的机制研究至关重要，但难以捉摸。在这里，我们使用实验和模拟来研究细胞与三种典型框架核酸 (FNA) 之间的相互作用，包括四面体、三棱柱和立方体。在这些 FNA 中观察到不同的细胞摄取效率，并且在多个细胞系中始终存在类似的区别。清道夫受体 (SRs) 被证明在介导摄取过程中是必不可少的。分子对接模拟显示，SR 结合主要取决于 FNA 的角角，决定了细胞内化频率。这项研究清楚地解释了 FNA 如何与膜相互作用以启动细胞进入，为治疗诊断纳米载体的设计和病毒入侵的研究提供了新的线索。

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