Name: EGFP (Enhanced Green Fluorescent Protein Recombinant)
Pricings: 5μg (RMB 300.00), 20μg (RMB 780.00), 1mg (RMB 11400.00)
Shipped: At Room Temperature
Catalogue Number: PRO-EGFP001
SYNONYMS
Green fluorescent protein, GFP.
INTRODUCTION
GFP, also known as Green Fluorescent Protein, is a protein produced by the jellyfish (Aequorea Victoria) that produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag. GFP is expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. Green Fluorescent Protein permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of proteinprotein interactions. Enhanced GFP (eGFP) has F64L and S65T mutations, which make GFP show increased fluorescence and fold more efficiently under 37 .
DESCRIPTION
Recombinant EGFP produced in E.coli cells is a nonglycosylated, homodimeric protein containing 239 amino acid chain and having a molecular mass of 26.9kDa. EGFP is purified by proprietary chromatographic techniques.
SOURCE
Escherichia Coli.
PHYSICAL APPEARANCE
Sterile Filtered White lyophilized (freezedried) powder.
FORMULATION
The EGFP was lyophilized from a 0.2μm filtered concentrated solution in PBS pH 7.4.
SOLUBILITY
It is recommended to reconstitute the lyophilized EGFP in sterile distilled H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
STABILITY
Lyophilized EGFP although stable at room temperature for 3 weeks, should be stored desiccated below 18℃. Upon reconstitution EGFP should be stored at 4℃ between 2-7 days and for future use below 18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freezethaw cycles.
PURITY
Greater than 95.0% as determined by: (a) Analysis by RPHPLC. (b) Analysis by SDSPAGE.
AMINO ACID SEQUENCE
MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTRAEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMVLLEFVTAAGI TLGMDELYK
USAGE
PuriMag's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
